Review



rnase v1  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher rnase v1
    Rnase V1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v1+rnase/Ribonuclease+A/pm41406077-234-40-46
    Average 99 stars, based on 1 article reviews
    rnase v1 - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: The Small Regulatory RNAs LhrC1–5 Contribute to the Response of Listeria monocytogenes to Heme Toxicity
    Article Snippet: Briefly, for the alkaline hydrolysis ladder, 0.2 pmol of labeled RNA was mixed with alkaline hydrolysis buffer (Ambion) and 10 μg of yeast tRNA (Ambion) in a total volume of 10 μL and incubated at 95°C for 5 min; for T1 control sample, 0.2 pmol of labeled RNA was denatured and incubated with 0.01 U of T1 RNase (Ambion) for 5 min. .. Structure probing RNA interactions were incubated at 37°C for 1 h before treating the samples with the indicated cleaving agent: 0.01 U T1 RNase for 5 min and 0.0015 U V1 RNase (Ambion) for 2 min. Control samples were prepared likewise (except for the cleaving agents) and incubated at 37°C for the duration of the experiment. .. Samples were placed on ice and mixed with 2× loading buffer type II (Ambion).

    Control:

    Article Title: The Small Regulatory RNAs LhrC1–5 Contribute to the Response of Listeria monocytogenes to Heme Toxicity
    Article Snippet: Briefly, for the alkaline hydrolysis ladder, 0.2 pmol of labeled RNA was mixed with alkaline hydrolysis buffer (Ambion) and 10 μg of yeast tRNA (Ambion) in a total volume of 10 μL and incubated at 95°C for 5 min; for T1 control sample, 0.2 pmol of labeled RNA was denatured and incubated with 0.01 U of T1 RNase (Ambion) for 5 min. .. Structure probing RNA interactions were incubated at 37°C for 1 h before treating the samples with the indicated cleaving agent: 0.01 U T1 RNase for 5 min and 0.0015 U V1 RNase (Ambion) for 2 min. Control samples were prepared likewise (except for the cleaving agents) and incubated at 37°C for the duration of the experiment. .. Samples were placed on ice and mixed with 2× loading buffer type II (Ambion).

    other:

    Article Title: Efficiency and precision of microRNA biogenesis modes in plants.
    Article Snippet: Partial RNA digestions were performed with T1 (Fermentas, native and denaturing conditions), S1 nuclease (Fermentas), V1 RNase (Ambion) and alkaline hydrolysis.

    Article Title: Efficiency and precision of miRNA biogenesis modes in plants
    Article Snippet: Partial RNA digestions were performed with T1 (Fermentas, native and denaturing conditions), S1 nuclease (Fermentas), V1 RNAse (Ambion) and alkaline hydrolysis.

    Purification:

    Article Title: Principles of miRNA/miRNA* function in plant MIRNA processing.
    Article Snippet: Using a T4 polynucleotide kinase (Thermo Fisher cientific), 100 pmol of dephosphorylated pri-miR394a and ri-miR319a transcripts were 5 ′ end labelled with [ γ - 32 P] TP (10 μCi / μl). .. The products were purified from a denaturng 8% (w / v) polyacrylamide gel using autoradiography and recipitated as described above. uclease digestions adioactively labelled products of in vitro transcription were artially digested using T1 RNAse (Fermentas, denaturing onditions), V1 RNase (Ambion, native conditions) and S1 uclease (Fermentas, native conditions) as described before 11 ). ..

    Autoradiography:

    Article Title: Principles of miRNA/miRNA* function in plant MIRNA processing.
    Article Snippet: Using a T4 polynucleotide kinase (Thermo Fisher cientific), 100 pmol of dephosphorylated pri-miR394a and ri-miR319a transcripts were 5 ′ end labelled with [ γ - 32 P] TP (10 μCi / μl). .. The products were purified from a denaturng 8% (w / v) polyacrylamide gel using autoradiography and recipitated as described above. uclease digestions adioactively labelled products of in vitro transcription were artially digested using T1 RNAse (Fermentas, denaturing onditions), V1 RNase (Ambion, native conditions) and S1 uclease (Fermentas, native conditions) as described before 11 ). ..

    In Vitro:

    Article Title: Principles of miRNA/miRNA* function in plant MIRNA processing.
    Article Snippet: Using a T4 polynucleotide kinase (Thermo Fisher cientific), 100 pmol of dephosphorylated pri-miR394a and ri-miR319a transcripts were 5 ′ end labelled with [ γ - 32 P] TP (10 μCi / μl). .. The products were purified from a denaturng 8% (w / v) polyacrylamide gel using autoradiography and recipitated as described above. uclease digestions adioactively labelled products of in vitro transcription were artially digested using T1 RNAse (Fermentas, denaturing onditions), V1 RNase (Ambion, native conditions) and S1 uclease (Fermentas, native conditions) as described before 11 ). ..



    Similar Products

    rnase v1  (Thermo Fisher)
    99
    Thermo Fisher rnase v1
    Rnase V1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v1+rnase/Ribonuclease+A/pm41406077-234-40-46
    Average 99 stars, based on 1 article reviews
    rnase v1 - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    v1 rnase  (Thermo Fisher)
    90
    Thermo Fisher v1 rnase
    Sequentially processed MIRNAs generate several small RNA duplexes with different structural requirements. ( A ) Scheme of a sequential MIRNA . The first DCL1 cut is highlighted with a black arrow. Small RNAs derived from sequential precursors. MiRNA is shown in red, distal miRNA duplex (miRNA.2) is depicted in light blue and proximal miRNA duplex (miRNA.1) is indicated in dark grey (right). ( B ) Top panel: logarithm in base 10 of number of reads of small RNA duplex (n.d.: not detected). See for the number of reads detected for each duplex. Bottom panel: dot plot representing |Δ G | calculated for each small RNA duplex from evolutionary conserved sequentially processed MIRNAs . The colour code of the small RNA duplexes is shown in (A). ( C ) Structural analysis of MIR319a . Denaturing 8% (w/v) polyacrylamide gels shown: OH−: alkaline hydrolysis; <t>V1:</t> <t>RNAse</t> V1 in decreasing concentrations; H2O: incubation with water (control). Blue lines indicate the double-stranded bases obtained after V1 digestion as determined from polyacrylamide gels. The arrows correlate the position of DCL1 cuts in the precursor (right) with the experimental determination of the secondary structure (left). See for the original image of the polyacrylamide gel. ( D, F and H ) Small RNA blots of transgenic lines expressing different precursors from the 35S promoter. Each sample corresponds to 15 pooled inflorescences from independent primary transgenic plants. Numbers above small RNA blots correspond to miRNA levels quantified relative to the wt precursor. The EB staining of each gel is shown below. Top panels show a schematic representation of MIR319a including the wt and modified sequences of (D) miR319.2 (miR319a.2GU and miR319a.2AU) (F) MIR319a-MIL where a black arrow indicates how the central internal loop in miRNA.1 duplex was moved closer to DCL1 second cut (F); and MIR319a-3MM wt and MIR319a-5MM (H). These depictions represent the mfold secondary structure prediction for each precursor (see Appendix II). Black letters specify the closed mismatches, and the numbers indicate their position counting from the 5′end of the miRNA or miRNA.2. MiRNA is shown in red and miRNA* in grey, miRNA.2 is shown in light blue, and miRNA.2* is shown in grey. |Δ G | is shown above each small RNA sequence. ( E , G and I ) RT-qPCR of pri-miRNA determined in three biological replicates are shown as triangles in different shades of grey. Lines represent the mean value for each MIRNA . No statistically significant differences in E, G and I. See for the statistical analysis conducted in all the samples.
    V1 Rnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v1+rnase/pmc11317159-77-16-18
    Average 90 stars, based on 1 article reviews
    v1 rnase - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Sequentially processed MIRNAs generate several small RNA duplexes with different structural requirements. ( A ) Scheme of a sequential MIRNA . The first DCL1 cut is highlighted with a black arrow. Small RNAs derived from sequential precursors. MiRNA is shown in red, distal miRNA duplex (miRNA.2) is depicted in light blue and proximal miRNA duplex (miRNA.1) is indicated in dark grey (right). ( B ) Top panel: logarithm in base 10 of number of reads of small RNA duplex (n.d.: not detected). See for the number of reads detected for each duplex. Bottom panel: dot plot representing |Δ G | calculated for each small RNA duplex from evolutionary conserved sequentially processed MIRNAs . The colour code of the small RNA duplexes is shown in (A). ( C ) Structural analysis of MIR319a . Denaturing 8% (w/v) polyacrylamide gels shown: OH−: alkaline hydrolysis; V1: RNAse V1 in decreasing concentrations; H2O: incubation with water (control). Blue lines indicate the double-stranded bases obtained after V1 digestion as determined from polyacrylamide gels. The arrows correlate the position of DCL1 cuts in the precursor (right) with the experimental determination of the secondary structure (left). See for the original image of the polyacrylamide gel. ( D, F and H ) Small RNA blots of transgenic lines expressing different precursors from the 35S promoter. Each sample corresponds to 15 pooled inflorescences from independent primary transgenic plants. Numbers above small RNA blots correspond to miRNA levels quantified relative to the wt precursor. The EB staining of each gel is shown below. Top panels show a schematic representation of MIR319a including the wt and modified sequences of (D) miR319.2 (miR319a.2GU and miR319a.2AU) (F) MIR319a-MIL where a black arrow indicates how the central internal loop in miRNA.1 duplex was moved closer to DCL1 second cut (F); and MIR319a-3MM wt and MIR319a-5MM (H). These depictions represent the mfold secondary structure prediction for each precursor (see Appendix II). Black letters specify the closed mismatches, and the numbers indicate their position counting from the 5′end of the miRNA or miRNA.2. MiRNA is shown in red and miRNA* in grey, miRNA.2 is shown in light blue, and miRNA.2* is shown in grey. |Δ G | is shown above each small RNA sequence. ( E , G and I ) RT-qPCR of pri-miRNA determined in three biological replicates are shown as triangles in different shades of grey. Lines represent the mean value for each MIRNA . No statistically significant differences in E, G and I. See for the statistical analysis conducted in all the samples.

    Journal: Nucleic Acids Research

    Article Title: Principles of miRNA/miRNA* function in plant MIRNA processing

    doi: 10.1093/nar/gkae458

    Figure Lengend Snippet: Sequentially processed MIRNAs generate several small RNA duplexes with different structural requirements. ( A ) Scheme of a sequential MIRNA . The first DCL1 cut is highlighted with a black arrow. Small RNAs derived from sequential precursors. MiRNA is shown in red, distal miRNA duplex (miRNA.2) is depicted in light blue and proximal miRNA duplex (miRNA.1) is indicated in dark grey (right). ( B ) Top panel: logarithm in base 10 of number of reads of small RNA duplex (n.d.: not detected). See for the number of reads detected for each duplex. Bottom panel: dot plot representing |Δ G | calculated for each small RNA duplex from evolutionary conserved sequentially processed MIRNAs . The colour code of the small RNA duplexes is shown in (A). ( C ) Structural analysis of MIR319a . Denaturing 8% (w/v) polyacrylamide gels shown: OH−: alkaline hydrolysis; V1: RNAse V1 in decreasing concentrations; H2O: incubation with water (control). Blue lines indicate the double-stranded bases obtained after V1 digestion as determined from polyacrylamide gels. The arrows correlate the position of DCL1 cuts in the precursor (right) with the experimental determination of the secondary structure (left). See for the original image of the polyacrylamide gel. ( D, F and H ) Small RNA blots of transgenic lines expressing different precursors from the 35S promoter. Each sample corresponds to 15 pooled inflorescences from independent primary transgenic plants. Numbers above small RNA blots correspond to miRNA levels quantified relative to the wt precursor. The EB staining of each gel is shown below. Top panels show a schematic representation of MIR319a including the wt and modified sequences of (D) miR319.2 (miR319a.2GU and miR319a.2AU) (F) MIR319a-MIL where a black arrow indicates how the central internal loop in miRNA.1 duplex was moved closer to DCL1 second cut (F); and MIR319a-3MM wt and MIR319a-5MM (H). These depictions represent the mfold secondary structure prediction for each precursor (see Appendix II). Black letters specify the closed mismatches, and the numbers indicate their position counting from the 5′end of the miRNA or miRNA.2. MiRNA is shown in red and miRNA* in grey, miRNA.2 is shown in light blue, and miRNA.2* is shown in grey. |Δ G | is shown above each small RNA sequence. ( E , G and I ) RT-qPCR of pri-miRNA determined in three biological replicates are shown as triangles in different shades of grey. Lines represent the mean value for each MIRNA . No statistically significant differences in E, G and I. See for the statistical analysis conducted in all the samples.

    Article Snippet: Radioactively labelled products of in vitro transcription were partially digested using T1 RNAse (Fermentas, denaturing conditions), V1 RNase (Ambion, native conditions) and S1 nuclease (Fermentas, native conditions) as described before ( ).

    Techniques: Derivative Assay, Incubation, Control, Transgenic Assay, Expressing, Staining, Modification, Sequencing, Quantitative RT-PCR